The Teamsters Rail Conference represents locomotive engineers, trainmen, conductors, track workers, bridge and building employees, and electric traction employees in some capacity on the following railroads:
 

Freight Railroads, USA

• Aliquippa and Southern

• Alleghany Ludlum Steel

• Belt Railway of Chicago

• Bessemer and Lake Erie Railroad

• Birmingham Southern

• Burlington Northern Santa Fe

• Conrail

• CP Rail System/U.S.

• CSX Transportation

• Dakota, Minnesota and Eastern

• Delaware & Hudson/Sprinfield Terminal Rwy.

• Duluth, Missabe and Iron Range

• Elgin, Joliet and Eastern

• Escanaba and Lake Superior Railroad

• Florida East Coast Railway

• Grand Trunk Western

• Great Western Railway of Colorado

• Guilford Transportation Industries

• Illinois Central

• Indiana & Ohio

• Indiana Harbor Belt

• Indiana Southern

• Iowa, Chicago & Eastern

• Iowa Interstate Railroad

• Kansas City Southern

• Lake Superior and Ishpeming Railroad

• Longview, Portland & Northern Rwy. & Longview Switching Co.

• Louisiana & Delta

• Montana Rail Link

• Missouri & Northern Arkansas

• New York & Atlantic

• New York, Susquehanna & Western

• Norfolk Southern

• Portland & Western

• St. Lawrence & Atlantic

• South Buffalo

• Tacoma Municipal Belt

• Terminal Railroad Association of St. Louis

• Texas Mexican

• Union Pacific

• Union Railroad

• Utah Railway

• Wisconsin Central 

Passenger Railroads, USA

• AMTRAK

• Long Island Rail Road

• METRA

• Metro North

• New Jersey Transit

• PATH (Port Authority Trans-Hudson Corp.)

• SEPTA